ABSTRACT
Thirty one thermophilic, celluloytic actinomycete isolates were selectively isolated from soil, compost, plant-waste biomass and ruminant manure samples, using Kosmachev liquid medium amended with 1% cellulose. Qulitative assessment of celluloytic acitivty was done measuring diameter of the clear zone by the growing bacterial colonies on soild medium containing cellulose. The relatively most active isolates were TA[10], TA[19] and TA[22]; subsequently were characterized and designated as Thermomonospora fusca Optimal growth conditions [time of incubation, temperature and pH] affecting cellulase complex production has been studied. Results showed that, optimal time for maxiarnl enzyme production was [192 216, 144]h. for isolates TA[10] and TA[22] respectively at an optimal temperature of [45°C], and pH values [8,9,9] respectively. Depletion of cellulose as the sole carbon and energy source, and the liberation of reducing sugars, were studied in relation with the cellular growth of the selected isolate TA[19], 810 mg/ml of reducing sugares were accumulated after 216h of incubation, with the concomitant consumption of 82% of cellulose after 240h. of growth
Subject(s)
Actinobacteria/growth & development , Actinobacteria/enzymology , Cellulases/biosynthesisABSTRACT
Extracellular cellulase crude enzyme complex preparation was obtaind. from the culture filtrate of Thermomonospora fusca isolate [TA[19]], grown in kosmachev liquid medium for [9] days at pH [9] and 45°C.Enzyme assays were carried out to determine the influence of time, temperature and pH required for maximal enzymatic activities of endoglucanase,exoglucanase and beta-glucosidase. Results showed that, higher enzyme activity was after [10,20,10] min at temperature [65,65,55°C] and pH [6] for mentioned enzymes, respectively